Part:BBa_K1582027
Thc_Cut1+inJanus-m Fusion Protein
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 982
Usage
This fusion protein is designed to degrade PET into terephthalic acid and ethylene glycol more powerfully. Compared to single Thc_Cut1, the rate of PET emzymolysis is supposed to be improved by Thc_Cut1-Janus fusion.
Biology
Thc_Cut1 is a kind of cutinase, the details of which can be seen at BBa_K1582028. Hydrophobin inJanus, from the edible mushroom Grifola frondosa, play a role in a broad range of processes in the growth and development of filamentous fungi. Their assembly could show rod-like structure. They can be expressed in eucaryotic cells. We did some mutations to it, in order to make it expressed in E.coli, and we call it inJanus-m.
According to some research, when Janus is added to the reaction of cutinase and plastics, the reaction rate is supposed to have an obvious improvement.
Based on the above background, we design to use the method of fusion protein to combine cutinase and Janus compactly, through the establishment of fusion protein, we can found if we can further promote the stimulation rate of the plastics degradation. Meanwhile, from the data about fusion protein, we can try to uncover the functional mechanism of the stimulation to the plastics degradation which is unknown until now.
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